2nd Place: 5-day-old zebrafish head (20X)
Dr. Hideo Otsuna
University of Utah Medical Center, Department of Neurobiology and Anatomy
Salt Lake City, Utah

Otsuna explains that this dorsal view of a 5-day-old zebrafish head shows tissue stained in blue, including the eyes, olfactory pits, forebrain, and midbrain. Green staining shows the neurons in the olfactory pits, forebrain, and elsewhere, while red shows retinal ganglion cells in the eyes and some midbrain neurons.

Otsuna makes such images in the course of his research. "I am making a 3D gene expression database in zebrafish head," he says, "this picture will go into a future database for NIH." Otsuna says he's a kind of imaging scientist: "Not perfectly artist and not perfectly biologist."
Courtesy of Nikon Small World / Hideo Otsuna